Journal: bioRxiv

Article Title: Investigating Mechanically Activated Currents from Trigeminal Neurons of Non-Human Primates

doi: 10.1101/2024.10.06.616876

Figure Lengend Snippet: Representative micro-photographs show expression patterns for CGRP (A) , trpV1 (B) , CGRP+trpV1 (C) , MrgprD (D) , tyrosine hydroxylase (E) and parvalbumin (F) in TG of adult male marmosets. Blue arrows on the panels A-C indicate CGRP + /trpV1 - neurons. Cyan arrows on the panels A-C show CGRP - /trpV1 + neurons. Antibodies used and matching colors are indicated. Scales are presented in each microphotograph. (G) Bar graphs reflect percentages of marker-positive sensory neurons in TG of adult male marmosets. (H) Bar graphs reflect relative percentages of CGRP + (peptidergic) and trpV1 + neurons in TG of adult male marmosets. X-axis denotes antibodies for markers. N=3.

Article Snippet: The following previously characterized primary antibodies were used: anti-CGRP guinea pig polyclonal (Synaptic Systems; Goettingen, Germany; catalog 414 004; 1:200) ( ); anti-TRPV1 rabbit polyclonal (Novus Biologicals; Centennial, CO; catalog NBP1-71774SS; 1:200) ( ); anti-mrgprD rabbit polyclonal (Alamone Lab; catalog ASR-031; 1:200) ( ; ); anti-tyrosine hydroxylase (TH) chicken polyclonal (Neuromics; Bloomington, MN; catalog CH23006; 1:300) ( ); anti-trkC rabbit polyclonal (Aviva Systems Biology, San Diego, CA; catalog ARP51318_P050; 1:200); anti-trkB goat polyclonal (R&D systems; AF1494; 1:200) ( ); anti-parvalbumin rabbit polyclonal (Novus Biologicals; catalogue NB120-11427SS; 1:200) ( ); and anti-calbindin D28k rabbit polyclonal (Synaptic Systems; catalogue 214 011; 1:200) ( ).

Techniques: Expressing, Marker

Journal: Bioengineering

Article Title: Formation and Long-Term Culture of hiPSC-Derived Sensory Nerve Organoids Using Microfluidic Devices

doi: 10.3390/bioengineering11080794

Figure Lengend Snippet: Characterization of neurospheres and axon bundles using fluorescent immunostaining. ( a , b ) TUJ1 as a neuron marker and ( a ) ISL1 as a sensory neuron marker. An organoid cultured for 39 days was used. ( b ) TRPV1 as a nociceptor marker. An organoid cultured for 28 days was used. White-colored scale bars: 500 μm. Gray-colored scale bars in close-up panels for axon bundles: 100 μm.

Article Snippet: For molecular characterization, the sensory nerve organoids were labeled with anti-tubulin β3 (TUBB3/TUJ1) mouse monoclonal antibody (MMS-435P, BioLegend, CA, USA), anti-ISL LIM homeobox 1 (ISL1) rabbit polyclonal antibodies (GTX102807, GeneTex, TX, USA), or anti-TRPV1 rabbit polyclonal antibodies (NBP1-97417, Novus Biologicals, CO, USA), followed by incubation with Alexa Fluor-conjugated anti-mouse or anti-rabbit polyclonal secondary antibodies (ab150109, ab150068, Abcam, Waltham, MA, USA).

Techniques: Immunostaining, Marker, Cell Culture

Journal: Journal of Oral Microbiology

Article Title: Toll-like receptors 2 and 4, and bacterial proteins in IgG4-related sialadenitis, other types of chronic sialadenitis and sialolithiasis

doi: 10.1080/20002297.2024.2382633

Figure Lengend Snippet: Staining patterns of TLR2, TLR4, gingipain R1 and LPS. (a) TLR2-positive sample from a patient with IgG4-positive chronic sclerosing sialadenitis. Intense staining is observed in the plasma cells (arrow) and the epithelial cells of the striate ducts (arrowhead). (b) TLR2-negative sample from a patient with IgG4-negative nonspecific chronic sialadenitis. (c) TLR4-positive sample from a patient with IgG4-positive sialadenitis. Intense positivity is observed in the plasma cells interspersing the fibrotic tissue (arrow) and the epithelial cells of the interlobular duct (arrowhead). (d) Negative TLR4 staining in a patient with IgG4-negative chronic sclerosing sialadenitis. Only the excretory material within the striate ducts is stained with TLR4 (asterisk). (e) Positive LPS staining in a patient with IgG4-positive chronic sclerosing sialadenitis. Staining positivity is seen exclusively in the mast cell-like cells (arrow). (f) Negative LPS staining in a patient with IgG4-RD. (g) Positive gingipain R1 staining in a patient with IgG4-positive chronic sclerosing sialadenitis. Weak staining positivity is present in inflammatory cells (arrow) and the epithelium of striate ducts (arrowhead). (h) Negative gingipain R1 staining in a patient with IgG4-RD. All photographs are taken at x400 magnification.

Article Snippet: For TLR2 staining, we used a rabbit polyclonal antihuman TLR2 antibody (NB100–56720, Novus Biologicals); for TLR4 staining, we used a mouse monoclonal antihuman TLR4 antibody (sc -293,072, Santa Cruz Biotechnology, Inc.); for LPS staining, we used a mouse monoclonal anti- E. coli LPS antibody (Abcam 35,654, Abcam); and for P. gingivalis gingipain R1 staining, we used a rabbit polyclonal anti- P. gingivalis gingipain R1 antibody (biorbyt orb243611, Biorybt).

Techniques: Staining, Clinical Proteomics

Journal: Journal of Oral Microbiology

Article Title: Toll-like receptors 2 and 4, and bacterial proteins in IgG4-related sialadenitis, other types of chronic sialadenitis and sialolithiasis

doi: 10.1080/20002297.2024.2382633

Figure Lengend Snippet: Distribution of gingipain R1 and LPS positivity between the IgG4-positive and the IgG4-negative groups. The Mann Whitney U-test was used to assess differences in the distribution of staining positivity between the IgG4-positive and the IgG4-negative groups. The significance level was set at p < 0.05.

Article Snippet: For TLR2 staining, we used a rabbit polyclonal antihuman TLR2 antibody (NB100–56720, Novus Biologicals); for TLR4 staining, we used a mouse monoclonal antihuman TLR4 antibody (sc -293,072, Santa Cruz Biotechnology, Inc.); for LPS staining, we used a mouse monoclonal anti- E. coli LPS antibody (Abcam 35,654, Abcam); and for P. gingivalis gingipain R1 staining, we used a rabbit polyclonal anti- P. gingivalis gingipain R1 antibody (biorbyt orb243611, Biorybt).

Techniques: MANN-WHITNEY, Staining

Journal: Journal of Oral Microbiology

Article Title: Toll-like receptors 2 and 4, and bacterial proteins in IgG4-related sialadenitis, other types of chronic sialadenitis and sialolithiasis

doi: 10.1080/20002297.2024.2382633

Figure Lengend Snippet: Gingipain R1 immunopositivity in the blood vessel endothelium (arrow) in a healthy control. Erythrocytes are seen inside the vessel (arrowhead). x400 magnification.

Article Snippet: For TLR2 staining, we used a rabbit polyclonal antihuman TLR2 antibody (NB100–56720, Novus Biologicals); for TLR4 staining, we used a mouse monoclonal antihuman TLR4 antibody (sc -293,072, Santa Cruz Biotechnology, Inc.); for LPS staining, we used a mouse monoclonal anti- E. coli LPS antibody (Abcam 35,654, Abcam); and for P. gingivalis gingipain R1 staining, we used a rabbit polyclonal anti- P. gingivalis gingipain R1 antibody (biorbyt orb243611, Biorybt).

Techniques: Control